Construct: shRNA TRCN0000066919

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009506.1-1113s1c1
DNA Barcode:
CCCTAATTCATGTGGAGCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vegfc (22341)
Gene Description:
vascular endothelial growth factor C
Transcript:
RefSeq NM_009506.1 (NON-CURRENT)
Match location:
Position 1113 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000066919)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTAATTCATGTGGAGCCAA
Hairpin Sequence:
5'-CCGG-CCCTAATTCATGTGGAGCCAA-CTCGAG-TTGGCTCCACATGAATTAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTAATTCATGTGGAGCCAACTCGAGTTGGCTCCACATGAATTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTAATTCATGTGGAGCCAACTCGAGTTGGCTCCACATGAATTAGGG-3'

Other clones with same target sequence:

(none)