Construct: shRNA TRCN0000067068

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010406.1-5124s1c1
DNA Barcode:
GCCTAGAATCAGATACACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hc (15139)
Gene Description:
hemolytic complement
Transcript:
RefSeq NM_010406.1 (NON-CURRENT)
Match location:
Position 5124 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067068)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTAGAATCAGATACACTTT
Hairpin Sequence:
5'-CCGG-GCCTAGAATCAGATACACTTT-CTCGAG-AAAGTGTATCTGATTCTAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTAGAATCAGATACACTTTCTCGAGAAAGTGTATCTGATTCTAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTAGAATCAGATACACTTTCTCGAGAAAGTGTATCTGATTCTAGGC-3'

Other clones with same target sequence:

(none)