Construct: shRNA TRCN0000067074

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007781.1-1556s1c1
DNA Barcode:
GCTCTCCACTTTGGCCGTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Csf2rb2 (12984)
Gene Description:
colony stimulating factor 2 receptor, beta 2, low-affinity (granulocyte-macrophage)
Transcript:
RefSeq NM_007781.1 (NON-CURRENT)
Match location:
Position 1556 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067074)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCTCCACTTTGGCCGTGTT
Hairpin Sequence:
5'-CCGG-GCTCTCCACTTTGGCCGTGTT-CTCGAG-AACACGGCCAAAGTGGAGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCTCCACTTTGGCCGTGTTCTCGAGAACACGGCCAAAGTGGAGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCTCCACTTTGGCCGTGTTCTCGAGAACACGGCCAAAGTGGAGAGC-3'

Other clones with same target sequence:

(none)