Construct: shRNA TRCN0000067123

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010580.1-2527s1c1
DNA Barcode:
CGCCTTCAACAAGTTCAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Itgb5 (16419)
Gene Description:
integrin beta 5
Transcript:
RefSeq NM_010580.1 (NON-CURRENT)
Match location:
Position 2527 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067123)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCTTCAACAAGTTCAACAA
Hairpin Sequence:
5'-CCGG-CGCCTTCAACAAGTTCAACAA-CTCGAG-TTGTTGAACTTGTTGAAGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCTTCAACAAGTTCAACAACTCGAGTTGTTGAACTTGTTGAAGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCTTCAACAAGTTCAACAACTCGAGTTGTTGAACTTGTTGAAGGCG-3'

Other clones with same target sequence:

  1. TRCN0000335578