Construct: shRNA TRCN0000067156

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031167.3-288s1c1
DNA Barcode:
TGGTGCCTATTGACCTTCATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Il1rn (16181)
Gene Description:
interleukin 1 receptor antagonist
Transcript:
RefSeq NM_031167.3 (NON-CURRENT)
Match location:
Position 288 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067156)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTGCCTATTGACCTTCATA
Hairpin Sequence:
5'-CCGG-TGGTGCCTATTGACCTTCATA-CTCGAG-TATGAAGGTCAATAGGCACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTGCCTATTGACCTTCATACTCGAGTATGAAGGTCAATAGGCACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTGCCTATTGACCTTCATACTCGAGTATGAAGGTCAATAGGCACCA-3'

Other clones with same target sequence:

  1. TRCN0000325759