Construct: shRNA TRCN0000067183

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_196586.3-639s1c1
DNA Barcode:
CCAGGAAATAAAGATCCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Igbp1 (18518)
Gene Description:
immunoglobulin (CD79A) binding protein 1
Transcript:
RefSeq XM_196586.3 (NON-CURRENT)
Match location:
Position 639 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067183)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGAAATAAAGATCCTGAA
Hairpin Sequence:
5'-CCGG-CCAGGAAATAAAGATCCTGAA-CTCGAG-TTCAGGATCTTTATTTCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGAAATAAAGATCCTGAACTCGAGTTCAGGATCTTTATTTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGAAATAAAGATCCTGAACTCGAGTTCAGGATCTTTATTTCCTGG-3'

Other clones with same target sequence:

  1. TRCN0000325761