Construct: shRNA TRCN0000067234

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009977.1-440s1c1
DNA Barcode:
CGGACTCTATATTGCTACTCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cst7 (13011)
Gene Description:
cystatin F (leukocystatin)
Transcript:
RefSeq NM_009977.1 (NON-CURRENT)
Match location:
Position 440 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067234)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGACTCTATATTGCTACTCT
Hairpin Sequence:
5'-CCGG-CGGACTCTATATTGCTACTCT-CTCGAG-AGAGTAGCAATATAGAGTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGACTCTATATTGCTACTCTCTCGAGAGAGTAGCAATATAGAGTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGACTCTATATTGCTACTCTCTCGAGAGAGTAGCAATATAGAGTCCG-3'

Other clones with same target sequence:

(none)