Construct: shRNA TRCN0000067307

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_484197.1-3086s1c1
DNA Barcode:
CGGGTTGCTTAAAGTTCTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Itgb8 (320910)
Gene Description:
integrin beta 8
Transcript:
RefSeq XM_484197.1 (NON-CURRENT)
Match location:
Position 3086 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067307)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGTTGCTTAAAGTTCTTAT
Hairpin Sequence:
5'-CCGG-CGGGTTGCTTAAAGTTCTTAT-CTCGAG-ATAAGAACTTTAAGCAACCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGTTGCTTAAAGTTCTTATCTCGAGATAAGAACTTTAAGCAACCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGTTGCTTAAAGTTCTTATCTCGAGATAAGAACTTTAAGCAACCCG-3'

Other clones with same target sequence:

(none)