Construct: shRNA TRCN0000067496

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013660.2-2144s1c1
DNA Barcode:
GCGGAACTCAAATGTTTCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sema4d (20354)
Gene Description:
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Transcript:
RefSeq NM_013660.2 (NON-CURRENT)
Match location:
Position 2144 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067496)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGAACTCAAATGTTTCCAA
Hairpin Sequence:
5'-CCGG-GCGGAACTCAAATGTTTCCAA-CTCGAG-TTGGAAACATTTGAGTTCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGAACTCAAATGTTTCCAACTCGAGTTGGAAACATTTGAGTTCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGAACTCAAATGTTTCCAACTCGAGTTGGAAACATTTGAGTTCCGC-3'

Other clones with same target sequence:

  1. TRCN0000312020