Construct: shRNA TRCN0000067751

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023052.1-398s1c1
DNA Barcode:
GCTGCAAGAGAACTGAACAGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccl21c (65956)
Gene Description:
chemokine (C-C motif) ligand 21C (leucine)
Transcript:
RefSeq NM_023052.1 (NON-CURRENT)
Match location:
Position 398 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067751)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCAAGAGAACTGAACAGA
Hairpin Sequence:
5'-CCGG-GCTGCAAGAGAACTGAACAGA-CTCGAG-TCTGTTCAGTTCTCTTGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCAAGAGAACTGAACAGACTCGAGTCTGTTCAGTTCTCTTGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCAAGAGAACTGAACAGACTCGAGTCTGTTCAGTTCTCTTGCAGC-3'

Other clones with same target sequence:

(none)