Construct: shRNA TRCN0000067757

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173422.1-499s1c1
DNA Barcode:
GAGGAGAAGAACTACAGGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Colec10 (239447)
Gene Description:
collectin sub-family member 10
Transcript:
RefSeq NM_173422.1 (NON-CURRENT)
Match location:
Position 499 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067757)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAGAAGAACTACAGGGAA
Hairpin Sequence:
5'-CCGG-GAGGAGAAGAACTACAGGGAA-CTCGAG-TTCCCTGTAGTTCTTCTCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAGAAGAACTACAGGGAACTCGAGTTCCCTGTAGTTCTTCTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAGAAGAACTACAGGGAACTCGAGTTCCCTGTAGTTCTTCTCCTC-3'

Other clones with same target sequence:

(none)