Construct: shRNA TRCN0000067789

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011124.3-217s1c1
DNA Barcode:
AGGAAGCAAGAACCAAGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccl21a (18829)
Gene Description:
chemokine (C-C motif) ligand 21A (serine)
Transcript:
RefSeq NM_011124.3 (NON-CURRENT)
Match location:
Position 217 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067789)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAAGCAAGAACCAAGTTTA
Hairpin Sequence:
5'-CCGG-AGGAAGCAAGAACCAAGTTTA-CTCGAG-TAAACTTGGTTCTTGCTTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAAGCAAGAACCAAGTTTACTCGAGTAAACTTGGTTCTTGCTTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAAGCAAGAACCAAGTTTACTCGAGTAAACTTGGTTCTTGCTTCCT-3'

Other clones with same target sequence:

(none)