Construct: shRNA TRCN0000067836

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009137.1-369s1c1
DNA Barcode:
TGGGTGAAGAAGCTACTCCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ccl22 (20299)
Gene Description:
chemokine (C-C motif) ligand 22
Transcript:
RefSeq NM_009137.1 (NON-CURRENT)
Match location:
Position 369 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067836)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGGTGAAGAAGCTACTCCAT
Hairpin Sequence:
5'-CCGG-TGGGTGAAGAAGCTACTCCAT-CTCGAG-ATGGAGTAGCTTCTTCACCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGGTGAAGAAGCTACTCCATCTCGAGATGGAGTAGCTTCTTCACCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGGTGAAGAAGCTACTCCATCTCGAGATGGAGTAGCTTCTTCACCCA-3'

Other clones with same target sequence:

(none)