Construct: shRNA TRCN0000067860

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008968.2-274s1c1
DNA Barcode:
CAGCTTCCTTACCAGGATGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptgis (19223)
Gene Description:
prostaglandin I2 (prostacyclin) synthase
Transcript:
RefSeq NM_008968.2 (NON-CURRENT)
Match location:
Position 274 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067860)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCTTCCTTACCAGGATGAA
Hairpin Sequence:
5'-CCGG-CAGCTTCCTTACCAGGATGAA-CTCGAG-TTCATCCTGGTAAGGAAGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCTTCCTTACCAGGATGAACTCGAGTTCATCCTGGTAAGGAAGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCTTCCTTACCAGGATGAACTCGAGTTCATCCTGGTAAGGAAGCTG-3'

Other clones with same target sequence:

(none)