Construct: shRNA TRCN0000067876

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_354628.1-231s1c1
DNA Barcode:
CTGTCGGTGTAAACCTGGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scarf1 (380713)
Gene Description:
scavenger receptor class F, member 1
Transcript:
RefSeq XM_354628.1 (NON-CURRENT)
Match location:
Position 231 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067876)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTCGGTGTAAACCTGGATT
Hairpin Sequence:
5'-CCGG-CTGTCGGTGTAAACCTGGATT-CTCGAG-AATCCAGGTTTACACCGACAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTCGGTGTAAACCTGGATTCTCGAGAATCCAGGTTTACACCGACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTCGGTGTAAACCTGGATTCTCGAGAATCCAGGTTTACACCGACAG-3'

Other clones with same target sequence:

(none)