Construct: shRNA TRCN0000067900

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008969.1-1582s1c1
DNA Barcode:
CCCAACTCCATCTTTGGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptgs1 (19224)
Gene Description:
prostaglandin-endoperoxide synthase 1
Transcript:
RefSeq NM_008969.1 (NON-CURRENT)
Match location:
Position 1582 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067900)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAACTCCATCTTTGGAGAA
Hairpin Sequence:
5'-CCGG-CCCAACTCCATCTTTGGAGAA-CTCGAG-TTCTCCAAAGATGGAGTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAACTCCATCTTTGGAGAACTCGAGTTCTCCAAAGATGGAGTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAACTCCATCTTTGGAGAACTCGAGTTCTCCAAAGATGGAGTTGGG-3'

Other clones with same target sequence:

(none)