Construct: shRNA TRCN0000067921

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013794.3-875s1c1
DNA Barcode:
GACTGGATAACTTCCCTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klra16 (27424)
Gene Description:
killer cell lectin-like receptor, subfamily A, member 16
Transcript:
RefSeq NM_013794.3 (NON-CURRENT)
Match location:
Position 875 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067921)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACTGGATAACTTCCCTCATT
Hairpin Sequence:
5'-CCGG-GACTGGATAACTTCCCTCATT-CTCGAG-AATGAGGGAAGTTATCCAGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACTGGATAACTTCCCTCATTCTCGAGAATGAGGGAAGTTATCCAGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACTGGATAACTTCCCTCATTCTCGAGAATGAGGGAAGTTATCCAGTC-3'

Other clones with same target sequence:

(none)