Construct: shRNA TRCN0000067984

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011786.1-1232s1c1
DNA Barcode:
GCACGAGAACAACACGCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aloxe3 (23801)
Gene Description:
arachidonate lipoxygenase 3
Transcript:
RefSeq NM_011786.1 (NON-CURRENT)
Match location:
Position 1232 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000067984)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACGAGAACAACACGCATTT
Hairpin Sequence:
5'-CCGG-GCACGAGAACAACACGCATTT-CTCGAG-AAATGCGTGTTGTTCTCGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACGAGAACAACACGCATTTCTCGAGAAATGCGTGTTGTTCTCGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACGAGAACAACACGCATTTCTCGAGAAATGCGTGTTGTTCTCGTGC-3'

Other clones with same target sequence:

(none)