Construct: shRNA TRCN0000068021

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008367.2-186s1c1
DNA Barcode:
CATTAACCATAGTACCCAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Il2ra (16184)
Gene Description:
interleukin 2 receptor, alpha chain
Transcript:
RefSeq NM_008367.2 (NON-CURRENT)
Match location:
Position 186 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068021)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTAACCATAGTACCCAGTT
Hairpin Sequence:
5'-CCGG-CATTAACCATAGTACCCAGTT-CTCGAG-AACTGGGTACTATGGTTAATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTAACCATAGTACCCAGTTCTCGAGAACTGGGTACTATGGTTAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTAACCATAGTACCCAGTTCTCGAGAACTGGGTACTATGGTTAATG-3'

Other clones with same target sequence:

(none)