Construct: shRNA TRCN0000068055

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008359.1-1478s1c1
DNA Barcode:
CCCAAGCAAAGTGGAAAGCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Il17ra (16172)
Gene Description:
interleukin 17 receptor A
Transcript:
RefSeq NM_008359.1 (NON-CURRENT)
Match location:
Position 1478 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068055)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGCAAAGTGGAAAGCTA
Hairpin Sequence:
5'-CCGG-CCCAAGCAAAGTGGAAAGCTA-CTCGAG-TAGCTTTCCACTTTGCTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGCAAAGTGGAAAGCTACTCGAGTAGCTTTCCACTTTGCTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGCAAAGTGGAAAGCTACTCGAGTAGCTTTCCACTTTGCTTGGG-3'

Other clones with same target sequence:

(none)