Construct: shRNA TRCN0000068078

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007648.1-149s1c1
DNA Barcode:
GCCGAGAACATTGAATACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cd3e (12501)
Gene Description:
CD3 antigen, epsilon polypeptide
Transcript:
RefSeq NM_007648.1 (NON-CURRENT)
Match location:
Position 149 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068078)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCGAGAACATTGAATACAAA
Hairpin Sequence:
5'-CCGG-GCCGAGAACATTGAATACAAA-CTCGAG-TTTGTATTCAATGTTCTCGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCGAGAACATTGAATACAAACTCGAGTTTGTATTCAATGTTCTCGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCGAGAACATTGAATACAAACTCGAGTTTGTATTCAATGTTCTCGGC-3'

Other clones with same target sequence:

(none)