Construct: shRNA TRCN0000068218

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009640.2-783s1c1
DNA Barcode:
CCCAGGTACTAAATCAAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Angpt1 (11600)
Gene Description:
angiopoietin 1
Transcript:
RefSeq NM_009640.2 (NON-CURRENT)
Match location:
Position 783 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068218)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGGTACTAAATCAAACAT
Hairpin Sequence:
5'-CCGG-CCCAGGTACTAAATCAAACAT-CTCGAG-ATGTTTGATTTAGTACCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGGTACTAAATCAAACATCTCGAGATGTTTGATTTAGTACCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGGTACTAAATCAAACATCTCGAGATGTTTGATTTAGTACCTGGG-3'

Other clones with same target sequence:

(none)