Construct: shRNA TRCN0000068287

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024470.1-162s1c1
DNA Barcode:
CCATAAGTCTTCAGGGTTGCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klra23 (79410)
Gene Description:
killer cell lectin-like receptor subfamily A, member 23
Transcript:
RefSeq NM_024470.1 (CURRENT)
Match location:
Position 162 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068287)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATAAGTCTTCAGGGTTGCA
Hairpin Sequence:
5'-CCGG-CCATAAGTCTTCAGGGTTGCA-CTCGAG-TGCAACCCTGAAGACTTATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATAAGTCTTCAGGGTTGCACTCGAGTGCAACCCTGAAGACTTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATAAGTCTTCAGGGTTGCACTCGAGTGCAACCCTGAAGACTTATGG-3'

Other clones with same target sequence:

(none)