Construct: shRNA TRCN0000068322

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355386.1-891s1c1
DNA Barcode:
CCGAGCATCAAAGGAAATCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC381424 (381424)
Gene Description:
similar to Ryanodine receptor 3 (Brain-type ryanodine receptor) (RyR3) (RYR-3) (Brain ryanodine receptor-calcium release channel)
Transcript:
RefSeq XM_355386.1 (NON-CURRENT)
Match location:
Position 891 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068322)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGCATCAAAGGAAATCAA
Hairpin Sequence:
5'-CCGG-CCGAGCATCAAAGGAAATCAA-CTCGAG-TTGATTTCCTTTGATGCTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGCATCAAAGGAAATCAACTCGAGTTGATTTCCTTTGATGCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGCATCAAAGGAAATCAACTCGAGTTGATTTCCTTTGATGCTCGG-3'

Other clones with same target sequence:

(none)