Construct: shRNA TRCN0000068325

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016984.1-1615s1c1
DNA Barcode:
GCAGAGGCATTATTTGCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trpc4 (22066)
Gene Description:
transient receptor potential cation channel, subfamily C, member 4
Transcript:
RefSeq NM_016984.1 (NON-CURRENT)
Match location:
Position 1615 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068325)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGAGGCATTATTTGCTATT
Hairpin Sequence:
5'-CCGG-GCAGAGGCATTATTTGCTATT-CTCGAG-AATAGCAAATAATGCCTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGAGGCATTATTTGCTATTCTCGAGAATAGCAAATAATGCCTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGAGGCATTATTTGCTATTCTCGAGAATAGCAAATAATGCCTCTGC-3'

Other clones with same target sequence:

(none)