Construct: shRNA TRCN0000068330

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008432.1-2617s1c1
DNA Barcode:
CCCTCCAACATCCACTTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnu1 (16532)
Gene Description:
potassium channel, subfamily U, member 1
Transcript:
RefSeq NM_008432.1 (NON-CURRENT)
Match location:
Position 2617 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068330)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTCCAACATCCACTTTATT
Hairpin Sequence:
5'-CCGG-CCCTCCAACATCCACTTTATT-CTCGAG-AATAAAGTGGATGTTGGAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTCCAACATCCACTTTATTCTCGAGAATAAAGTGGATGTTGGAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTCCAACATCCACTTTATTCTCGAGAATAAAGTGGATGTTGGAGGG-3'

Other clones with same target sequence:

(none)