Construct: shRNA TRCN0000068357

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_358019.1-95s1c1
DNA Barcode:
CACATGTCAATATGCACAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC385052 (385052)
Gene Description:
similar to Voltage-dependent calcium channel gamma-7 subunit (Neuronal voltage-gated calcium channel gamma-7 subunit)
Transcript:
RefSeq XM_358019.1 (NON-CURRENT)
Match location:
Position 95 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068357)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACATGTCAATATGCACAGAA
Hairpin Sequence:
5'-CCGG-CACATGTCAATATGCACAGAA-CTCGAG-TTCTGTGCATATTGACATGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACATGTCAATATGCACAGAACTCGAGTTCTGTGCATATTGACATGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACATGTCAATATGCACAGAACTCGAGTTCTGTGCATATTGACATGTG-3'

Other clones with same target sequence:

(none)