Construct: shRNA TRCN0000068365

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031169.2-210s1c1
DNA Barcode:
CCAGGAATCCATATGTCACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnmb1 (16533)
Gene Description:
potassium large conductance calcium-activated channel, subfamily M, beta member 1
Transcript:
RefSeq NM_031169.2 (NON-CURRENT)
Match location:
Position 210 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068365)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGAATCCATATGTCACTT
Hairpin Sequence:
5'-CCGG-CCAGGAATCCATATGTCACTT-CTCGAG-AAGTGACATATGGATTCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGAATCCATATGTCACTTCTCGAGAAGTGACATATGGATTCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGAATCCATATGTCACTTCTCGAGAAGTGACATATGGATTCCTGG-3'

Other clones with same target sequence:

(none)