Construct: shRNA TRCN0000068371

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011390.1-3013s1c1
DNA Barcode:
TGGACGAAAGAGAAACTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc12a7 (20499)
Gene Description:
solute carrier family 12, member 7
Transcript:
RefSeq NM_011390.1 (NON-CURRENT)
Match location:
Position 3013 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068371)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGACGAAAGAGAAACTCATT
Hairpin Sequence:
5'-CCGG-TGGACGAAAGAGAAACTCATT-CTCGAG-AATGAGTTTCTCTTTCGTCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGACGAAAGAGAAACTCATTCTCGAGAATGAGTTTCTCTTTCGTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGACGAAAGAGAAACTCATTCTCGAGAATGAGTTTCTCTTTCGTCCA-3'

Other clones with same target sequence:

  1. TRCN0000327498