Construct: shRNA TRCN0000068423

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011406.1-2011s1c1
DNA Barcode:
GCCCTGTTGTTGAATGAGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc8a1 (20541)
Gene Description:
solute carrier family 8 (sodium/calcium exchanger), member 1
Transcript:
RefSeq NM_011406.1 (NON-CURRENT)
Match location:
Position 2011 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068423)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTGTTGTTGAATGAGCTT
Hairpin Sequence:
5'-CCGG-GCCCTGTTGTTGAATGAGCTT-CTCGAG-AAGCTCATTCAACAACAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTGTTGTTGAATGAGCTTCTCGAGAAGCTCATTCAACAACAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTGTTGTTGAATGAGCTTCTCGAGAAGCTCATTCAACAACAGGGC-3'

Other clones with same target sequence:

(none)