Construct: shRNA TRCN0000068449

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133207.1-873s1c1
DNA Barcode:
CCCAAGAACATATTTAGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnh7 (170738)
Gene Description:
potassium voltage-gated channel, subfamily H (eag-related), member 7
Transcript:
RefSeq NM_133207.1 (NON-CURRENT)
Match location:
Position 873 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068449)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAAGAACATATTTAGAGAT
Hairpin Sequence:
5'-CCGG-CCCAAGAACATATTTAGAGAT-CTCGAG-ATCTCTAAATATGTTCTTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAAGAACATATTTAGAGATCTCGAGATCTCTAAATATGTTCTTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAAGAACATATTTAGAGATCTCGAGATCTCTAAATATGTTCTTGGG-3'

Other clones with same target sequence:

(none)