Construct: shRNA TRCN0000068461

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012035.1-1126s1c1
DNA Barcode:
GCAACAGTCTATCGCTGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trpc7 (26946)
Gene Description:
transient receptor potential cation channel, subfamily C, member 7
Transcript:
RefSeq NM_012035.1 (NON-CURRENT)
Match location:
Position 1126 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068461)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAACAGTCTATCGCTGTGAA
Hairpin Sequence:
5'-CCGG-GCAACAGTCTATCGCTGTGAA-CTCGAG-TTCACAGCGATAGACTGTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAACAGTCTATCGCTGTGAACTCGAGTTCACAGCGATAGACTGTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAACAGTCTATCGCTGTGAACTCGAGTTCACAGCGATAGACTGTTGC-3'

Other clones with same target sequence:

(none)