Construct: shRNA TRCN0000068466

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175090.2-169s1c1
DNA Barcode:
GCACCACCATATGGGTATGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc31a1 (20529)
Gene Description:
solute carrier family 31, member 1
Transcript:
RefSeq NM_175090.2 (NON-CURRENT)
Match location:
Position 169 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068466)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCACCATATGGGTATGTA
Hairpin Sequence:
5'-CCGG-GCACCACCATATGGGTATGTA-CTCGAG-TACATACCCATATGGTGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCACCATATGGGTATGTACTCGAGTACATACCCATATGGTGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCACCATATGGGTATGTACTCGAGTACATACCCATATGGTGGTGC-3'

Other clones with same target sequence:

(none)