Construct: shRNA TRCN0000068470

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007384.1-759s1c1
DNA Barcode:
CCTGCTCTACTGGCTCAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Asic2 (11418)
Gene Description:
acid-sensing (proton-gated) ion channel 2
Transcript:
RefSeq NM_007384.1 (NON-CURRENT)
Match location:
Position 759 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068470)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCTCTACTGGCTCAGTTT
Hairpin Sequence:
5'-CCGG-CCTGCTCTACTGGCTCAGTTT-CTCGAG-AAACTGAGCCAGTAGAGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCTCTACTGGCTCAGTTTCTCGAGAAACTGAGCCAGTAGAGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCTCTACTGGCTCAGTTTCTCGAGAAACTGAGCCAGTAGAGCAGG-3'

Other clones with same target sequence:

(none)