Construct: shRNA TRCN0000068496

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355877.1-579s1c1
DNA Barcode:
GTGGCTATATTCTTCCTGGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnk6 (52150)
Gene Description:
potassium inwardly-rectifying channel, subfamily K, member 6
Transcript:
RefSeq XM_355877.1 (NON-CURRENT)
Match location:
Position 579 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068496)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGCTATATTCTTCCTGGTA
Hairpin Sequence:
5'-CCGG-GTGGCTATATTCTTCCTGGTA-CTCGAG-TACCAGGAAGAATATAGCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGCTATATTCTTCCTGGTACTCGAGTACCAGGAAGAATATAGCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGCTATATTCTTCCTGGTACTCGAGTACCAGGAAGAATATAGCCAC-3'

Other clones with same target sequence:

(none)