Construct: shRNA TRCN0000068545

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011399.1-463s1c1
DNA Barcode:
CCAACTAACTACAAAGGCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc25a17 (20524)
Gene Description:
solute carrier family 25 (mitochondrial carrier, peroxisomal membrane protein), member 17
Transcript:
RefSeq NM_011399.1 (NON-CURRENT)
Match location:
Position 463 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068545)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACTAACTACAAAGGCATT
Hairpin Sequence:
5'-CCGG-CCAACTAACTACAAAGGCATT-CTCGAG-AATGCCTTTGTAGTTAGTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACTAACTACAAAGGCATTCTCGAGAATGCCTTTGTAGTTAGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACTAACTACAAAGGCATTCTCGAGAATGCCTTTGTAGTTAGTTGG-3'

Other clones with same target sequence:

(none)