Construct: shRNA TRCN0000068591

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020574.3-613s1c1
DNA Barcode:
CATCAAGAACCGTGTGTCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcne3 (57442)
Gene Description:
potassium voltage-gated channel, Isk-related subfamily, gene 3
Transcript:
RefSeq NM_020574.3 (NON-CURRENT)
Match location:
Position 613 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068591)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCAAGAACCGTGTGTCTAT
Hairpin Sequence:
5'-CCGG-CATCAAGAACCGTGTGTCTAT-CTCGAG-ATAGACACACGGTTCTTGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCAAGAACCGTGTGTCTATCTCGAGATAGACACACGGTTCTTGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCAAGAACCGTGTGTCTATCTCGAGATAGACACACGGTTCTTGATG-3'

Other clones with same target sequence:

(none)