Construct: shRNA TRCN0000068657

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021452.1-126s1c1
DNA Barcode:
CCCGCCTTGCAGGATCTGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnmb4 (58802)
Gene Description:
potassium large conductance calcium-activated channel, subfamily M, beta member 4
Transcript:
RefSeq NM_021452.1 (CURRENT)
Match location:
Position 126 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068657)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGCCTTGCAGGATCTGCAA
Hairpin Sequence:
5'-CCGG-CCCGCCTTGCAGGATCTGCAA-CTCGAG-TTGCAGATCCTGCAAGGCGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGCCTTGCAGGATCTGCAACTCGAGTTGCAGATCCTGCAAGGCGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGCCTTGCAGGATCTGCAACTCGAGTTGCAGATCCTGCAAGGCGGG-3'

Other clones with same target sequence:

(none)