Construct: shRNA TRCN0000068682

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_148929.2-999s1c1
DNA Barcode:
GCATCGTGATGTCACACTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc9a8 (77031)
Gene Description:
solute carrier family 9 (sodium/hydrogen exchanger), member 8
Transcript:
RefSeq NM_148929.2 (NON-CURRENT)
Match location:
Position 999 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068682)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCGTGATGTCACACTATA
Hairpin Sequence:
5'-CCGG-GCATCGTGATGTCACACTATA-CTCGAG-TATAGTGTGACATCACGATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCGTGATGTCACACTATACTCGAGTATAGTGTGACATCACGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCGTGATGTCACACTATACTCGAGTATAGTGTGACATCACGATGC-3'

Other clones with same target sequence:

(none)