Construct: shRNA TRCN0000068728

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013927.1-3864s1c1
DNA Barcode:
CGGAGACAATTACCAAAGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cngb3 (30952)
Gene Description:
cyclic nucleotide gated channel beta 3
Transcript:
RefSeq NM_013927.1 (NON-CURRENT)
Match location:
Position 3864 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068728)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGACAATTACCAAAGTTA
Hairpin Sequence:
5'-CCGG-CGGAGACAATTACCAAAGTTA-CTCGAG-TAACTTTGGTAATTGTCTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGACAATTACCAAAGTTACTCGAGTAACTTTGGTAATTGTCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGACAATTACCAAAGTTACTCGAGTAACTTTGGTAATTGTCTCCG-3'

Other clones with same target sequence:

(none)