Construct: shRNA TRCN0000068790

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_129721.4-2035s1c1
DNA Barcode:
CCAGGCGAAATGGATGAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc9a2 (226999)
Gene Description:
solute carrier family 9 (sodium/hydrogen exchanger), member 2
Transcript:
RefSeq XM_129721.4 (NON-CURRENT)
Match location:
Position 2035 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068790)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGCGAAATGGATGAAATT
Hairpin Sequence:
5'-CCGG-CCAGGCGAAATGGATGAAATT-CTCGAG-AATTTCATCCATTTCGCCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGCGAAATGGATGAAATTCTCGAGAATTTCATCCATTTCGCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGCGAAATGGATGAAATTCTCGAGAATTTCATCCATTTCGCCTGG-3'

Other clones with same target sequence:

(none)