Construct: shRNA TRCN0000068812

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177353.2-964s1c1
DNA Barcode:
CCATTGTACTATCCTCGTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc9a7 (236727)
Gene Description:
solute carrier family 9 (sodium/hydrogen exchanger), member 7
Transcript:
RefSeq NM_177353.2 (NON-CURRENT)
Match location:
Position 964 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068812)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATTGTACTATCCTCGTCTA
Hairpin Sequence:
5'-CCGG-CCATTGTACTATCCTCGTCTA-CTCGAG-TAGACGAGGATAGTACAATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATTGTACTATCCTCGTCTACTCGAGTAGACGAGGATAGTACAATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATTGTACTATCCTCGTCTACTCGAGTAGACGAGGATAGTACAATGG-3'

Other clones with same target sequence:

(none)