Construct: shRNA TRCN0000068865

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027782.1-392s1c1
DNA Barcode:
CCTCAAGGCAATTACTTCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kctd6 (71393)
Gene Description:
potassium channel tetramerisation domain containing 6
Transcript:
RefSeq NM_027782.1 (NON-CURRENT)
Match location:
Position 392 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068865)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCAAGGCAATTACTTCATT
Hairpin Sequence:
5'-CCGG-CCTCAAGGCAATTACTTCATT-CTCGAG-AATGAAGTAATTGCCTTGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCAAGGCAATTACTTCATTCTCGAGAATGAAGTAATTGCCTTGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCAAGGCAATTACTTCATTCTCGAGAATGAAGTAATTGCCTTGAGG-3'

Other clones with same target sequence:

(none)