Construct: shRNA TRCN0000068870

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_132686.3-435s1c1
DNA Barcode:
CAAAGATGTTAAAGACCTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc41a3 (71699)
Gene Description:
solute carrier family 41, member 3
Transcript:
RefSeq XM_132686.3 (NON-CURRENT)
Match location:
Position 435 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068870)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGATGTTAAAGACCTGAT
Hairpin Sequence:
5'-CCGG-CAAAGATGTTAAAGACCTGAT-CTCGAG-ATCAGGTCTTTAACATCTTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGATGTTAAAGACCTGATCTCGAGATCAGGTCTTTAACATCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGATGTTAAAGACCTGATCTCGAGATCAGGTCTTTAACATCTTTG-3'

Other clones with same target sequence:

(none)