Construct: shRNA TRCN0000068888

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009134.1-6145s1c1
DNA Barcode:
GTGTTGTTTGTGACCAGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scn10a (20264)
Gene Description:
sodium channel, voltage-gated, type X, alpha
Transcript:
RefSeq NM_009134.1 (NON-CURRENT)
Match location:
Position 6145 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068888)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTTGTTTGTGACCAGAGAT
Hairpin Sequence:
5'-CCGG-GTGTTGTTTGTGACCAGAGAT-CTCGAG-ATCTCTGGTCACAAACAACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTTGTTTGTGACCAGAGATCTCGAGATCTCTGGTCACAAACAACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTTGTTTGTGACCAGAGATCTCGAGATCTCTGGTCACAAACAACAC-3'

Other clones with same target sequence:

(none)