Construct: shRNA TRCN0000068892

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009134.1-433s1c1
DNA Barcode:
CCATATTTATCACTGTCACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scn10a (20264)
Gene Description:
sodium channel, voltage-gated, type X, alpha
Transcript:
RefSeq NM_009134.1 (NON-CURRENT)
Match location:
Position 433 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068892)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATATTTATCACTGTCACTA
Hairpin Sequence:
5'-CCGG-CCATATTTATCACTGTCACTA-CTCGAG-TAGTGACAGTGATAAATATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATATTTATCACTGTCACTACTCGAGTAGTGACAGTGATAAATATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATATTTATCACTGTCACTACTCGAGTAGTGACAGTGATAAATATGG-3'

Other clones with same target sequence:

(none)