Construct: shRNA TRCN0000068928
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_130259.4-933s1c1
- DNA Barcode:
- CCCTGGAATCAAGCTGATCTA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Slc43a1 (72401)
- Gene Description:
- solute carrier family 43, member 1
- Transcript:
- RefSeq XM_130259.4 (NON-CURRENT)
- Match location:
- Position 933 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000068928)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCCTGGAATCAAGCTGATCTA
- Hairpin Sequence:
-
5'-CCGG-CCCTGGAATCAAGCTGATCTA-CTCGAG-TAGATCAGCTTGATTCCAGGG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCCTGGAATCAAGCTGATCTACTCGAGTAGATCAGCTTGATTCCAGGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCCTGGAATCAAGCTGATCTACTCGAGTAGATCAGCTTGATTCCAGGG-3'
Other clones with same target sequence:
(none)
