Construct: shRNA TRCN0000068940

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138655.1-2150s1c1
DNA Barcode:
CCGAGTTTGATATTAGTGGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmc2 (192140)
Gene Description:
transmembrane channel-like gene family 2
Transcript:
RefSeq NM_138655.1 (CURRENT)
Match location:
Position 2150 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000068940)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGTTTGATATTAGTGGAA
Hairpin Sequence:
5'-CCGG-CCGAGTTTGATATTAGTGGAA-CTCGAG-TTCCACTAATATCAAACTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGTTTGATATTAGTGGAACTCGAGTTCCACTAATATCAAACTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGTTTGATATTAGTGGAACTCGAGTTCCACTAATATCAAACTCGG-3'

Other clones with same target sequence:

(none)