Construct: shRNA TRCN0000068943
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_141275.4-711s1c1
- DNA Barcode:
- GCCTATGGATTTCAAACCTAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC241422 (241422)
- Gene Description:
- similar to Sodium channel protein type III alpha subunit (Voltage-gated sodium channel alpha subunit Nav1.3) (Sodium channel protein, brain III alpha subunit) (Voltage-gated sodium channel subtype III)
- Transcript:
- RefSeq XM_141275.4 (NON-CURRENT)
- Match location:
- Position 711 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000068943)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCCTATGGATTTCAAACCTAT
- Hairpin Sequence:
-
5'-CCGG-GCCTATGGATTTCAAACCTAT-CTCGAG-ATAGGTTTGAAATCCATAGGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCCTATGGATTTCAAACCTATCTCGAGATAGGTTTGAAATCCATAGGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCCTATGGATTTCAAACCTATCTCGAGATAGGTTTGAAATCCATAGGC-3'
Other clones with same target sequence:
(none)
