Construct: shRNA TRCN0000069010

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_136482.3-103s1c1
DNA Barcode:
CGGAGAGTTAAGATCAACGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnb2 (98741)
Gene Description:
potassium voltage gated channel, Shab-related subfamily, member 2
Transcript:
RefSeq XM_136482.3 (NON-CURRENT)
Match location:
Position 103 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000069010)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAGAGTTAAGATCAACGTA
Hairpin Sequence:
5'-CCGG-CGGAGAGTTAAGATCAACGTA-CTCGAG-TACGTTGATCTTAACTCTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAGAGTTAAGATCAACGTACTCGAGTACGTTGATCTTAACTCTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAGAGTTAAGATCAACGTACTCGAGTACGTTGATCTTAACTCTCCG-3'

Other clones with same target sequence:

(none)